采用响应面法优化旧报纸的裂褶菌粗酶液脱墨

Optimization of Schizophyllum Commune Deinking of Waste Newspaper by Response Surface Methodology

关键词:  响应面法  裂褶菌  粗酶液  脱墨

Key Words:response surface methodology  Schizophyllum Commune  crude enzymes  deinking

摘要:利用裂褶菌（Schizophyllum Commune）在限氮培养条件下产生的粗酶液对旧报纸进行脱墨，采用响应面法中的Box-Behnken实验设计，对影响脱墨的主要因素酶用量、温度和pH值进行优化，得到了用于预测脱墨浆有效残余油墨浓度（ERIC值）和白度值的数学模型。模型方差分析和响应面曲线分析表明，pH值对裂褶菌粗酶液脱墨效果的影响最为显著。优化结果显示，当酶用量0.20 IU/g、温度52.5℃和pH值6.5时，脱墨浆有效残余油墨浓度最低，为208.0 mg/kg；当酶用量0.24 IU/g、温度52.2℃和pH值6.5时，脱墨浆白度最高，为53.3%。验证实验证明了模型优化的准确性。

Abstract:The deinking of waste newspaper with the crude enzymes produced by Schizophyllum commune in nutrient nitrogen limited culture medium was studied. Box-Behnken design in Response Surface Methodology was employed to optimize the main factors including enzymes dosage, temperature and pH which influence the deinking result, and two mathematic models were proposed to predict the effective residual ink concentration (ERIC) and brightness of the pulp. The experiment results showed that pH had more obvious influence on Schizophyllum Commune deinking. The lowest ERIC of the pulp 208.0 mg/kg was reached while the deinking parameters were as follows: enzymes dosage 0.20 IU/g, temperature 52.5℃ and pH 6.5. When enzymes dosage, temperature and pH were 0.24 IU/g, 52.2℃ and 6.5 respectively, the brightness of the pulp peaked at 53.3%ISO. Comparing the experimental data and predicted date, the models were confirmed to be of high precision.