固相萃取-高效液相色谱法测定纸品中的丙烯酰胺

Determination of Acrylamide in Paper by Solid-phase Extraction and HPLC

关键词:  固相萃取  高效液相色谱法  纸品  丙烯酰胺  测定

Key Words:solid-phase extraction  HPLC  paper  acrylamide  determination

摘要:建立固相萃取-高效液相色谱法测定纸品中丙烯酰胺含量的方法。样品中的丙烯酰胺经超纯水在60℃的条件下提取，离心分离，0.45 μm滤膜过滤并浓缩后，采用固相萃取柱进行净化，然后通过高效液相色谱进行分析测定。该方法的检出限为10 μg/kg，具有操作方便、准确率高、重复性好等优点，可用于各类纸品中丙烯酰胺含量的检测。

Abstract:Solid-phase extraction and HPLC were applied to the determination of acrylamide in paper. Acrylamide in paper was extracted with ultrapure water at 60℃, the aqueous extract was centrifuged and filtrated with 0.45 μm filter membrane, and then concentrated by Nitrogen evaporators. The concentrated liquid was purified by solid-phase extraction (SPE) and determined by HPLC. The limit of detection (LOD) of the developed method was 10 μg/kg. This method has the advantages of convenient operation, high sensitivity, excellent accuracy and good reproducibility and is therefore suitable for the determination of acrylamide in paper.