利用半纤维素水解液中的木糖生物合成壳聚糖

Biosynthesis of Chitosan Using Xylose from Hemicellulose Hydrolysate

关键词:  壳聚糖  半纤维素  制浆废液  脱毒  雅致放射毛霉

Key Words:chitosan  hemicellulose  pulping effluent  detoxification  Actinomucor elegans

摘要:研究了制浆废液中半纤维素水解液中的木糖用于生物合成壳聚糖的脱毒及培养条件。结果表明,最优脱毒条件为：先加入Ca(OH)2调pH值至10,抽滤后再加入质量分数10%的H2SO4调pH值至7,再次抽滤后以1∶20的固液比加入活性炭,置于50℃摇床以120 r/min的转速处理2 h,抽滤后取上清液。雅致放射毛霉(Actinomucor elegansTCCC450005)生物合成壳聚糖的最佳培养条件为：以6%的玉米浆为氮源,以适当稀释(使木糖浓度为3%)的脱毒后制浆废液为碳源,接入雅致放射毛霉,于30℃下培养60 h,菌丝体浓度可达15.20 g/L,壳聚糖产量为2.02 g/L。

Abstract:The detoxification technology and culture conditions of biosynthesis of chitosan using xylose from hemicelluloses hydrolysate such as pulping waste liquor were investigated. The detoxification technology was as follows: pH was adjusted to 10 with Ca(OH)2 at first then adjusted back to 7 with 10% H2SO4 after suction filtration, then activated carbon was added in a ratio of 1∶20(W∶V) after the second suction filtration, finally it was put on a rocking table at the tempreture of 50℃ and the rotating speed of 120 r/min for 2 hours, the supernatant after the final suction filtration was collected for cultivation. The culture conditions included that using 6% of corn milk as nitrogen source and properly diluted detoxicated pulping waste liquor (which contains 3% of xylose) as carbon source. Actinomucor elegans(TCCC450005) was cultured in this medium at 30℃ for 60 hours. The mycelia concentration was 15.20 g/L and the chitasan yield was 2.02 g/L.