粉单竹竹青色素提取工艺及其成分分析

Extraction Technology and Composition Analysis of Bamboo Bark Pigment from BambusaChungii

关键词:  粉单竹竹青  色素  超声波提取

Key Words:BambusaChungii bamboo bark  pigment  ultrasonic extraction

摘要:以乙醇为提取溶剂，采用超声波提取粉单竹竹青色素，通过单因素实验，获得竹青色素最佳提取条件为：乙醇浓度90%，料液比1∶35，超声时间40 min,超声温度55℃，提取两次。实验结果表明，在最佳提取条件下粉单竹竹青叶绿素提取率为0.39%；色素纯化后通过柱层析分离得到橙黄色、蓝绿色、黄色、绿色4种色素，在紫外可见光区（400~800 nm）测4种色素紫外光谱图，发现该图形分别与叶绿素a、叶绿素b和两种类胡萝卜素的谱图相似；通过核磁共振分析，纯化后的提取物中可能含有脂肪酸，甾醇等；通过红外光谱分析可知，提取液中含有羟基、CC双键、CO双键、C—O键等官能团。

Abstract:Bamboo bark pigment was extracted from BambusaChungii by ultrasonic extraction, using ethanol as extraction solvent. The optimum extraction conditions were as the follows: ethanol concentration 90%, solid liquid ratio 1∶35, ultrasonic time 40 min, ultrasonic temperature 55℃. After twice extraction under the optimum condition, the pigment was basically extracted completely, the extraction rate of chlorophyll was about 0.39%. Pigment was purified and separation by column chromatography to obtain orange pigment, turquoise pigment, yellow pigment and green pigment,then graphics of Uv vis spectrophotometer were similar to chlorophyll a, chlorophyll b and two carotenoids, and the purified extracting may also contain fatty acids, sterols and so on identific by nuclear magnetic spectrum(NMR), the purified extracting contained —OH, CC, CO, C—O and so on by fourier transform infrared spectroscopy(FT-IR) analysis.