表面活性剂改善高固体系木质纤维素酶水解的研究

Study on the Improvement of Enzymatic Hydrolysis of Lignocellulose by Surfactant Under High Solid System

关键词:  高固体系  酶水解  木质素磺酸钠  表面活性剂

Key Words:high solid system  enzymatic hydrolysis  sodium lignosulfonate  surfactant

摘要:本研究以木质素磺酸钠为主要助剂，考察其对经金属氯盐预处理的桉木粉在高固体系纤维素酶水解中的影响。结果表明，木质素磺酸钠的加入对高固体系的酶水解效率有较大促进作用，当木质素磺酸钠加入量为0.4%时，酶水解效率可达93.72%，与未添加样相比，酶水解效率提高了12.5个百分点。为进一步提高酶水解效率，将木质素磺酸钠分别与CTAB（十六烷基三甲基溴化铵）和吐温80两种表面活性剂复配，结果表明，表面活性剂复配对高固体系的酶水解效率均有协同促进作用；当m(CTAB)∶m(木质素硫酸钠)为1.5∶1时，酶水解效率可达99.56%；m(吐温80)∶m(木质素磺酸钠)为1∶1时，酶水解效率为99.98%，纤维素基本可完全水解为还原糖。

Abstract:In this research， sodium lignosulfonate was used as an assistant to improve the enzymatic hydrolysis efficiency of Eucalyptus powders pretreated with metal chloride under high solid system. The results showed that the addition of sodium lignosulfonate promoted the enzymatic hydrolysis efficiency of the high solid system. When the amount of sodium lignosulfonate was 0.4%， the glucose yield from enzymatic hydrolysis could reach to 93.72%， which was 12.5 percent higher than that of blank sample. In order to further improve the efficiency of enzymatic hydrolysis， sodium lignosulfonate was compounded with CTAB and Tween 80. The results showed that the efficiency of enzymatic hydrolysis of high solid system was promoted synergistically. When m （CTAB）∶m （sodium lignosulfonate） was 1.5∶1， the cellulose conversion rate was 99.56%； when m （Tween 80）∶m （sodium lignosulfonate） was 1∶1， the cellulose conversion rate was 99.98%， so nearly total of the cellulose could be converted to glucose.