化学竹浆废水活性污泥细菌菌群结构分析

Bacterial Community Composition of Activated Sludge from Chemical Bamboo Pulp Wastewater

关键词:  化学竹浆废水  生物处理  活性污泥  细菌群落  高通量测序

Key Words:chemical bamboo pulp wastewater  biological treatment  activated sludge  bacterial community  pyrosequencing

摘要:本研究利用超高通量测序技术分析实际化学竹浆废水活性污泥中的细菌菌群结构和多样性，分析表明，不同竹浆废水处理厂废水香农指数为4.48~5.19，ACE指数为767.80~1066.15，进一步得到不同化学竹浆废水活性污泥细菌菌群不仅具备多样性，同时具有较为显著的差异性。结果表明，化学竹浆废水活性污泥中比较常见的菌门包括Proteobacteria（变形菌门）、Chloroflexi（绿弯菌门）、Bacteroidetes（拟杆菌门）和Actinobacteria（放线菌门），其中Proteobacteria（变形菌门）占主导地位，最高丰度为59.47%；主要属为Hyphomicrobium（生丝微菌属）、Candidatus_Competibacter、JdFR-76和SWB02，其中生丝微菌属占主导地位，丰度为3.69%~18.29%。这些菌属不仅与硝化和反硝化过程相关，还与多环芳香族类化合物、醇类及烷烃类物质的降解直接有关。

Abstract:In this study， the structure and diversity of bacterial community in activated sludge of actual chemical bamboo pulping wastewater were analyzed by using ultra-high throughput sequencing technology. The shannon index and ACE index ranged from 4.48 to 5.19 and 767.80 to 1066.15 respectively， which indicated the activated sludge bacterial communities in different chemical bamboo pulping wastewater were not only diversity but also significant differentiation. The classification of common bacteria in activated sludge of chemical bamboo pulping wastewater included： Proteobacteria， Chloroflexi， Bacteroidetes and Actinobacteria. Proteobacteria was the dominant phylum and its maximum abundance was 59.47%. The primary genera were Hyphomicrobium， Candidatus_Competibacter， JdFR-76 and SWB02. Hyphomicrobium dominated with an abundance of 3.69%~18.29%. These bacteria were not only related to nitrification and denitrification， but also directly related to the degradation of polycyclic aromatic compounds， alcohols and alkanes.