海藻酸钠-琼脂-蒙脱土固定化菌对造纸废水降酚性能的研究

Study on the Degradation Performance of Sodium Alginate-Agar-Montmorillonite Immobilized Bacteria on Reducing Phenol in Papermaking Wastewater

关键词:  蒙脱土  海藻酸钠-琼脂  降酚菌  固定化  降解动力学

Key Words:montmorillonite  sodium alginate-agar  phenol-degrading bacteria  immobilization  degradation kinetics

摘要:本研究利用有机和无机耦合材料包埋固定化苯酚降解菌构建固定化微球，研究了固定化微球对造纸废水中苯酚的生物降解能力。结果表明，细菌Klebsiella pneumoniae ZS01固定最优条件为：1.0%海藻酸钠，1.5%蒙脱土，1.0%琼脂，25%细菌接种量，苯酚初始浓度为1000 mg/L。此时固定化微球的苯酚生物降解率（16.67 mg/（L·h））显著高于游离细菌（13.89 mg/（L·h））。进一步研究发现在高苯酚浓度、强酸和强碱环境下，固定化微球降解苯酚效果显著高于游离态细菌。在对苯酚降解动力学的研究中发现霍尔丹模型与实验数据吻合较好，说明高浓度苯酚对细菌生物降解有较大的抑制作用。固定化微球重复使用32次，苯酚降解效率依然高达99.5%；在 4℃下储存30天时，固定化微球的苯酚降解率保持在99%以上。

Abstract:In this paper， organic and inorganic coupling materials were used to embed immobilized phenol-degrading bacteria to construct immobilized microspheres， and the biodegradability of immobilized phenol-degrading bacteria on phenol in papermaking wastewater was studied. The results showed that the optimal conditions for the fixation of the bacteria Klebsiella pneumoniae ZS01 were： 1.0% sodium alginate， 1.5% montmorillonite， 1.0% agar， 25% bacterial inoculum， and the initial phenol concentration of 1000 mg/L. The phenol biodegradation rate of immobilized micropheres （16.67 mg/（L·h）） was significantly higher than that of free bacteria （13.89 mg/（L·h））. Further research found that under high phenol concentration， strong acid and strong alkali environment， the effect of immobilized micropheres to degrade phenol was significantly higher than that of free bacteria. In the study of phenol degradation kinetics， it was found that Haldane's model was in good agreement with the experimental data， indicating that high concentrations of phenol had a greater inhibitory effect on bacterial biodegradation. The immobilized micropheres were reused 32 times， and the phenol degradation efficiency was still as high as 99.5%， and stored at 4°C for 30 days， the phenol reduction efficiency of the immobilized micropheres remained above 99%.