钙化对生产性厌氧反应器中颗粒污泥特性的影响

Effect of Calcification on the Characteristics of Granular Sludge in Anaerobic Reactor

关键词:  废纸制浆废水  钙化  颗粒污泥  微生物群落

Key Words:waste paper pulping wastewater  calcification  granular sludge  microbial community

摘要:本研究以某废纸制浆企业的钙化厌氧反应器为背景，跨年度采集了厌氧颗粒污泥样本，系统分析了其理化和生物特性的差异。结果表明，钙化过程中颗粒污泥内碳酸钙持续累积，导致Ca²⁺含量、灰分增加，并加快了颗粒沉降速度，同时伴随着污泥粒径增加。长期暴露于高钙废水中，细菌群落中产氢产乙酸菌的相对丰度显著提升，而水解酸化菌的丰度显著降低；古菌群落中，原有优势菌Methanobacterium（产甲烷杆菌属）减少，而适应新环境的Bathyarchaeia、Methanolinea（产甲烷八叠球菌属）和Candidatus_Methanofastidiosum等菌群显著增加。通过解析钙化对颗粒污泥特性及微生物群落的影响，可为解析生产性反应器中高钙环境对颗粒污泥响应提供相关信息。

Abstract:This study investigated a calcified anaerobic reactor at a waste paper pulping company， where granular samples were collected over a year. Systematic analysis of the physicochemical and biological properties of the granules revealed that calcium carbonate accumulation during calcification led to increased Ca²⁺ content， higher ash content， accelerated settling velocity and larger diameter. Prolonged exposure to high-calcium wastewater significantly enhanced the relative abundance of hydrogen-producing acetogens in the bacterial community， while the abundance of hydrolytic bacteria and acidogens declined. In the archaeal community， the original dominant methanogens Methanobacterium decreased， while archaea adapted to the high-calcium environment showed significant growth， such as Bathyarchaeia， Methanolinea， and Candidatus_Methanofastidiosum. These changes in physicochemical properties and microbial community succession provided critical insights into the response mechanisms of granular sludge under calcification stress.