离子色谱-积分脉冲安培法在纸浆纤维单糖组成分析中的应用

Application of Ion Chromatography-Integrated Pulsed Amperometric Method in the Analysis of Monosaccharide Composition of Pulp Fiber

关键词:  离子色谱  积分脉冲安培法  纸浆纤维  水解  单糖组成

Key Words:ion chromatography  integrated pulsed amperometric detection  pulp fiber  hydrolysis  monosaccharide composition

摘要:本研究建立了离子色谱-积分脉冲安培法检测纸浆纤维水解液中单糖（阿拉伯糖、半乳糖、葡萄糖、木糖和甘露糖）、糖醛酸（半乳糖醛酸和葡萄糖醛酸）和纤维二糖的方法。采用积分脉冲安培检测器，用氢氧化钠和醋酸钠为洗脱液，在CarboPAC PA 10（2.0 mm×250 mm）阴离子交换柱上进行梯度洗脱，分离得到8种糖组分。结果表明，在浓度0.01～1.0 µg/mL范围内，8种糖组分线性关系良好，相关系数R²>0.9988，检出限为0.0022～0.0046 mg/L，且峰面积的相对标准偏差（RSD）在0.63%～1.83%之间，平均回收率为96.62%～102.42%。应用该方法分析纤维的水解单糖组成，发现云杉纤维、桉木纤维、竹纤维、亚麻纤维、龙须草纤维和棉纤维的酸水解产物中均不含半乳糖醛酸和葡萄糖醛酸，且单糖组成差异也较大。

Abstract:In this study, an analytical method for the determination of monosaccharide composition (arabinose, galactose, glucose, xylose and mannose), uronic acid（galacturonic acid, glucuronic acid） and cellobiose in the pulp fibers hydrolysate was established by ion chromatography coupled with integrated pulsed amperometric detection.The analysis was performed on an anion-exchange column CarboPac PA 10 (2.0 mm × 250 mm) with gradient elution of NaOH and NaOAc as mobile phases, and the sugars were separated by integrated pulsed amperometric detector. The eight sugars showed a good linear relationship and the correlation coefficient R² of linear regression equations were above 0.9988 in the concentration range of 0.01～1.0 µg/mL, the detection limit was 0.0022～0.0046 mg/L, the relative standard deviation (RSD) was 0.63%～1.83%, and the average recovery was 96.62%～102.42%, which met the requirements of analysis and detection. The monosaccharide composition of several pulp fibers in papermaking was further determined by this method, which showed that the hydrolysates of fibers from spruce, eucalyptus, bamboo, flax, Chinese alpine rush and cotton were free of galacturonic acid and glucuronic acid, and their monosaccharide compositions were quite different.